Part:BBa_K5222010

CDS of HsOMT adapted for B3-B5 position of MoClo ( codon optimized for chlamydomonas reinhardtii)

In the article Biological Valorization of Poly(ethylene terephthalate) Monomers for Upcycling Waste PET, the authors identified a Homo sapiens O-methyltransferase (HsOMT) enzyme as the most efficient protein for converting protocatechuic acid (PCA) into vanillic acid (VA). Ana Rita Brochado et al. also reported that HsOMT plays a crucial role in vanillin production and that overexpressing this enzyme in a strain with model-guided modifications can significantly increase the final product yield.

Essentially, this enzyme catalyses the methylation reaction, where a methyl group is added to the hydroxyl group on the PCA molecule, converting it into vanillic acid. Here we created a CDS of HsOMT adapted for B3-B5 position of MoClo and optimized for for chlamydomonas reinhardtii

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 37
  Illegal XhoI site found at 90
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]